FoUUi . ATCC stands ready to support our customers’ needs during the coronavirus pandemic. Loucy and OCI-AML1 cell lines. The cell lines are promising … Cell Line Selector; Downloads; About . Status. LOUCY and HL60 cell lines were cultured in the absence or presence of DMSO (vehicle control) or KPT-330 (100 nM or 1000 nM) for 24 hr; cell lysates were prepared using RIPA buffer and analyzed by immunoblotting with anti-Nup214, anti-CRM1, or anti-GAPDH antibodies. 513 genes with high or low expression in LOUCY relative to other cell lines from the GDSC Cell Line Gene Expression Profiles dataset. $21.00, For-Profit: Disease Leukemia Disease Subtype Acute Lymphoblastic Leukemia (ALL), T-cell … Although these cytogenetic tests revealed complex karyotypes, such as additional copies of chromosome 13 in PL-21, FLT3 mutations could still be detected and their allele frequencies effectively calculated. (B–D) CRM1 ChIP-seq for HL60 cells was performed as described in Figure 1. On the other hand, other T cell lines as well as a human mononuclear cell line, THP-1, expressed BAFF almost constitutively . P53 gene mutation in a T-acute lymphoblastic leukemia cell line (loucy) with t(16:20) and 5q- chromosomal aberrations. To study the anti-apoptotic BCL-2 dependencies we will use the cutting-edge technology mitochondrial BH3 profiling. From ESCAPE . The line was established by Nakabayshi, H. and Sato, J. Huh-7 is highly susceptible to the hepatitis C virus (HCV), and it is often used as a model to investigate hepatoma and HCV. 83 genes mutated in the LOUCY cell line from the CCLE Cell Line Gene Mutation Profiles dataset. LOUCY_HAEMATOPOIETIC_AND_LYMPHOID_TISSUE Lineage: T-cell_ALL. Expression of the α-subunit of the interleukin-2 receptor (IL-2Rα) was assessed by quantifying CD25 upregulation in the Jurkat cell line (human acute T cell leukemia). The LOUCY T-ALL cell line has been shown to have a transcriptional signature similar to early immature primary T-ALL cells, as compared with several more-differentiated T-ALL cell lines… Of special note is that the formation of the SET exon 7/NUP214 exon 18 gene transcript requires alternative splicing as the SET breakpoint is located downstream of the stop codon in exon 8. We have updated our. $21.00 Compared with the C and NC groups, overexpression of ROCK2 significantly upregulated lncRNA AWPPH expression (P<0.05; Fig. Among T cell lines thus far examined, a T cell line named Loucy, which was previously established from T cell acute lymphoblastic leukemia , showed robust induction of BAFF in response to the stimulation. K562, HL60, and HEL cells were obtained from JCRB cell bank (Ibaraki, Osaka). The operation of this screen utilizes 60 different human tumor cell lines, representing leukemia, melanoma and cancers of the lung, colon, brain, ovary, breast, prostate, and kidney cancers. All Rights Reserved. sets of target genes of transcription factors from published ChIP-chip, ChIP-seq, and other transcription factor binding site profiling studies;sets of differentiall Cell lines (LOUCY, MOLT-16, PEER, and ALL-SIL) were cultured in the presence of nelarabine or the abovementioned drugs, either alone or in combination at a fixed ratio (Fig.   |   Our aim was to investigate whether Loucy cell line shares similarities with γδ T cells derived from human peripheral blood mononuclear cells (PBMC). Such bioactive compounds are associated with chemopreventive actions in several cancer cell lines. $49.00 Description Established in 1987 from the peripheral blood of a patient with T-cell acute lymphoblastic leukemia (T-ALL) obtained two months prior to her death. LOUCY. On the other hand, other T cell lines as well as a human mononuclear cell line, THP-1, expressed BAFF almost constitutively .   |   Disease Leukemia Disease Subtype Acute Lymphoblastic Leukemia (ALL), T-cell … 18th Mar, 2015. These STRs were confirmed to be biallelic in each cell line using cytogenetic and FISH analyses. T-cell acute lymphoblastic leukemia (T-ALL) arises from the malignant transformation of T-cell progenitors at various stages of development. Other Notes Tissue: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. $49.00. Depmap ID: ACH-000104. Dataset: COSMIC Cell Line Gene CNV Profiles: Category: genomics Type: cell line: Description : disease: T lymphocyte; acute lympho- blastic leukemia; t(16;20) translocation (Cell Line Ontology, CLO_0007375) Similar Terms: Downloads & Tools: Genes. Of special note is that the formation of the SET exon 7/NUP214 exon 18 gene transcript requires alternative splicing as the SET breakpoint is located downstream of the stop codon in exon 8. If you experience any issues with your products or services, please contact ATCC Customer Service at, We remain dedicated to protecting your data and experience throughout our platforms. Product ID CRL-2629. Depmap ID: ACH-000937. If you have questions about this, please contact us at info@biocompare.com. PubMed: 9643574. In myeloid and ALL cell lines, ABT-737 was shown to increase sensitivity toward chemotherapeutic agents. Viewing mutations requires login and acceptance of the Terms of access. It is important for genetic studies. STAT1 was also phosphorylated in the LOUCY and TALL-1 TYK2-independent cell lines, presumably due to activation by other pathways, indicating that these cell lines do not depend on the TYK2 pathway for survival. Cellosaurus RRID: CVCL_1641. Murine Embryo Fibroblast cell lines 3T3-L1 1 Murine Embryo Fibroblast cell line  /  When a cell line possesses conflicted markers, the different combinations of alleles are computed and the Best and Worst reported as distinct rows with an additional label after their accession number. Epithelial Cells, More Characteristics. $614.00, For-Profit: Chemical structures of (A) tubacin and (B) its inactive carboxylic acid analog, niltubacin. Product 282 genes mutated in the LOUCY cell line from the COSMIC Cell Line Gene Mutation Profiles dataset. This cell line may be of value in evaluating the role of t(16;20) in the etiology of T-ALL. Home sets of target genes of transcription factors from published ChIP-chip, ChIP-seq, and other transcription factor binding site profiling studies;sets of differentiall Mutations for Cell line LOUCY | associated with blood bone marrow, haematopoietic and lymphoid tissue, leukaemia, lymphoid neoplasm | canSAR First, 95% of Loucy cells express the unique γδ T cell receptor (TCR). IC 50 = 2 μ M for Jurkat cells, IC 50 = 1.2 μ M for Loucy cells, IC 50 = 1.2 μ M for Nalm-6 cells, and IC 50 = 1.4 μ M for REH cells. CCLE Name: LOUCY_HAEMATOPOIETIC_AND_LYMPHOID_TISSUE. Term ID EFO:0007112. Non-Profit: PubMed: 2208060, Prokocimer M, et al. Organism: Among T cell lines thus far examined, a T cell line named Loucy, which was previously established from T cell acute lymphoblastic leukemia , showed robust induction of BAFF in response to the stimulation. A human T-acute lymphoblastic leukemia (ALL) cell line (Loucy), derived from cells from a patient with resistant ALL with a t(16:20) and 5q- chromosomal aberrations was evaluated for p53 gene alterations and expression. An email will be sent to you with instructions. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Ben-Bassat H, et al. Contact Us Cite. May be of value in evaluating the role of t(16;20) in the etiology of T-ALL. Although THP-1 cells are of the same lineage, mutations can cause differences as the progeny proliferates. Growth Properties: Small, round cells growing Mostly singly. For lentiviral transduction, 10 5 cells were spinfected with MISSION shRNA lentiviral transduction particles (Sigma) according to the manufacturer’s protocol, at a multiplicity of infection of 1. ISO 9001 T lymphocyte DSMZ no. A human T-acute lymphoblastic leukemia (ALL) cell line (Loucy), derived from cells from a patient with resistant ALL with a t(16:20) and 5q- chromosomal aberrations was evaluated for p53 gene alterations and expression. Citation Guide: When you publish your research, please cite our product as “AcceGen Biotech Cat.# released. (Loucy cell line) and typical T-ALL (CEM-CCRF cell line) if distinct microenvironments affect the BCL-2 family proteins expression and the sensitivity to BH3 mimetics. Cancer Genet. Formats Price. The ATCC Cell Biology Collection is one of the largest bioresources in the world, and offers a complex array of human, animal, insect, fish and stem cell lines from which to choose. LOUCY Gene Set. $614.00 4a). Similar Cell lines for LOUCY | blood bone marrow, haematopoietic and lymphoid tissue, leukaemia, lymphoid neoplasm COSMIC, the Catalogue Of Somatic Mutations In Cancer, is the world's largest and most comprehensive resource for exploring the impact of … 4A). Södersjukhuset (Stockholm South General Hospital) We have been developing a … Conclusions . 49: 241-248, 1990. LOUCY sequenced and profiled in following datasets Omics Characterizations Expression Public 20Q3 Copy Number Public 20Q3 Mutation Public 20Q3 Fusion 20Q3 Protein Array Methylation (1kb upstream TSS) Copy Number (Absolute) Loucy; ENCBS170DPY / cell line { ; } Summary. DepMap; DepMap Consortium; Resources; Publications; Funders; depmap@broadinstitute.org @CancerDepMap; Forum; PF382. Sanger ID: 1043. Please stand by, while we are checking your browser... Biocompare is the leading resource for up-to-date product information, product reviews, Summary Homo sapiens Loucy cell line. peripheral blood Cell lines BE-13 and PEER show identical DNA fingerprints and hence share common origin, presumably due to cross-contamination. Mutations for Cell line LOUCY | associated with blood bone marrow, haematopoietic and … Luciferase-positive LOUCY cells were generated by infecting the LOUCY cell line with a PWPI-LUC lentivirus. Aliases: Loucy. 40,41 In addition, a strong synergism between ABT-199 and cytarabine has been previously reported in the T-ALL cell line LOUCY. The line was established by Nakabayshi, H. and Sato, J. Huh-7 is highly susceptible to the hepatitis C virus (HCV), and it is often used as a model to investigate hepatoma and HCV. K562, Jurkat, PEER, and LOUCY cell lines were maintained in RPMI medium containing 10% fetal calf serum and 50 μM 2-mercaptoethanol. Bone … Western blot analysis of p53 showed elevated levels of the protein. From ESCAPE . … Cell Bank Australia reference number: CBA1341 Culture Medium RPMI 1640 (with 2mM L-Glutamine+25mM HEPES) + 15% FCS Subculture Routine Optimal split ratio 1:4 - 1:8 (seeding density 0.8 x104 cells/cm 2).Cells incubated at 37°C with 5% CO 2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.  /  Quantity Add to cart. Second, most Loucy cells were double negative for … Special offers and promos that will save your lab money! Aliases: PF-382. The HuH-7 cell line was established in 1982 from a well differentiated hepatocyte derived cellular carcinoma cell line that was originally taken from a liver tumor in a 57-year-old Japanese male. © 1999-2017 Biocompare. Therefore, we performed a reverse transcriptase (RT)-PCR based screening of 141 leukemia/lymphoma cell lines of T-, B- and myeloid cell origin to detect SET-NUP214 positive examples. Other Notes Click for Delivery Prices. The HuH-7 cell line was established in 1982 from a well differentiated hepatocyte derived cellular carcinoma cell line that was originally taken from a liver tumor in a 57-year-old Japanese male. All rights reserved. Sources Cell Line LOUCY atcc-LOUCY cansar-LOUCY dsmz-LOUCY expasy-LOUCY CCLE-LOUCY Aliases --- --- Type T-ALL --- Patient Data Female, 38 years old T cell acute lymphoblastic leukemia T cell leukemia from peripheral blood Ben-Bassat1990 Immuno-phenotype CD3+ CD4- CD8- Sandberg2007(Table1) Peirs2014(SupTable1) TCR Rearrangement Status ɣδ Sandberg2007(Table1) Peirs2014(SupTable1) … (C) Effects of tubacin treatment for 72 h on proliferation of lymphoblastic leukemia cell lines. LOUCY_HAEMATOPOIETIC_AND_LYMPHOID_TISSUE Lineage: T-cell_ALL. Disease: This screen is unique in that the complexity of a 60 cell line dose response produced by a given compound results in a biological response pattern which can be utilized in pattern recognition … Product ID CRL … Reverse tran … : ACC 394. The NCI-60 Human Tumor Cell Lines Screen has served the global cancer research community for >20 years. Establishment of a human T-acute lymphoblastic leukemia cell line with a (16;20) chromosome translocation. 2B and 2C).   |     |   Your account has been locked. Using our custom target enrichment design, we were able to generate sequence reads covering all coding bases of FLT3 to a minimum read depth of 80x (average=2793x; median=1120x). Our Technical Support team may be able to help. Lymphoma 29: 607-611, 1998. CCLE Name: PF382_HAEMATOPOIETIC_AND_LYMPHOID_TISSUE. Leukemia cell lines, LOUCY, MEGAL, and OCI-AML3 were purchased from DSMZ (Braunschweig, Germany). Species: human ( Homo sapiens) Cell type: T cell leukemia. The screen was implemented in fully operational form in 1990 and utilizes 60 different human tumor cell lines to identify and characterize novel compounds with growth inhibition or killing of tumor cell lines. The aim is to stain human gingival epithelial cells with this occludin antibody COSMIC ID: 907789. Cell Line Selector; Downloads; About . Homo sapiens, human It is important for genetic studies. Sources Cell Line MOLT-16 cansar-MOLT16 dsmz-MOLT16 expasy-MOLT16 igrcid-MOLT16 CCLE-MOLT16 Aliases --- --- Type T-ALL --- Patient Data Female, 5 years old T cell acute lymphoblastic leukemia T cell leukemia from peripheral blood Drexler1989 Immuno-phenotype CD3+ CD4- CD8- Minowada1989 Burger1999 Sandberg2007(Table1) TCR Rearrangement Status αβ Minowada1989 … Cell lines are useful model systems to elucidate the cellular function of oncogenes. Non-Profit: Panel of Mouse cell lines CLS Cell Lines Service Part of the CLS cell bank Table 1: Mouse cell lines: Origin and General Characteristics Name of cell line Cell type Organism Strain Age / Gender Tissue / Disease Morphology Growth properties CLS order no. Before proceeding, please verify that you are a real user. NCI-60 Human Tumor Cell Lines Screen Introduction. Frozen. Figure 2. 821 genes with high or low expression in LOUCY relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset. The Loucy cell line was established in 1987 from the peripheral blood of a patient with T-cell acute lymphoblastic leukemia (T-ALL) obtained two months prior to her death To ATCC Valued Customers, ATCC stands ready to support our customers’ needs during the coronavirus pandemic. The Loucy cell line was established in 1987 from the peripheral blood of a patient with T-cell acute lymphoblastic leukemia (T-ALL) obtained two months prior to her death [53286] This cell line may be of value in evaluating the role of t(16;20) in the etiology of T-ALL. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Compound sensitivity for Cell line LOUCY | associated with blood bone marrow, haematopoietic and lymphoid tissue, leukaemia, lymphoid neoplasm | canSAR © 2020 ATCC. The Loucy cell line was established in 1987 from the peripheral blood of a patient with T-cell acute lymphoblastic leukemia (T-ALL) obtained two months prior to her death, OKT3+, OKT4+, THB4+, J5 +/-, OKT6-, TdT-, and HLA-DR-, Ethical Standards for Obtaining Human Materials, Nucleic Acids, Proteins and Cell Extracts, p53 + overexpressed; (mutated, GTG --> ATG mutation at codon 272), The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC, Cultures can be maintained by addition of fresh medium or replacement of medium. £505.0. Enter your user name and click Submit. high expression. We also performed XTT assay of single drug components nelfinavir, everolimus and palbociclib and confirmed higher efficacy on LOUCY cell line (Fig. The Loucy cell line was established in 1987 from the peripheral blood of a patient with T-cell acute lymphoblastic leukemia (T-ALL) obtained two months prior to her death [53286] This cell line may be of value in evaluating the role of t(16;20) in the etiology of T-ALL. Careers P53 gene mutation in a T-acute lymphoblastic leukemia cell line (loucy) with t(16:20) and 5q- chromosomal aberrations. Are you sure? Article Title: ZEB2 drives immature T-cell lymphoblastic leukaemia development via enhanced tumour-initiating potential and IL-7 receptor signalling Article Snippet: ..Luciferase-positive LOUCY cells were generated by infecting the LOUCY cell line with a PWPI-LUC lentivirus. Privacy Policy Origin: established from the peripheral blood of a 38-year-old woman with T cell acute lymphoblastic leukemia, T-ALL (FAB L2) (chemotherapy-resistant, remission was never achieved); cell line expresses the SET-NUP214 fusion transcript. T-cell acute lymphoblastic leukemia, For-Profit: ATCC was entrusted with its first cell line in 1962 and has consistently attained the highest standards and used the most reliable procedures to verify every cell line since. Cell Lines Project v92, released 27-AUG-20. I have screened Jurkat (E6 clone) and HuT 78 from ATCC and unfortunely the levels of CD are very low. 3B). The HeLa cell line gave them the time and the possibility to conduct repeatable experiments on human cells, without testing directly on humans. 282 genes mutated in the LOUCY cell line from the COSMIC Cell Line Gene Mutation Profiles dataset. By switching countries your current shopping cart will be cleared. Cytogenet. LOUCY, MEGAL, and HL60 cells were cultured in RPMI1640 medium (Sigma) … It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein interactions, and immunohistochemistry. Please view the Terms & Conditions of Supply for more information. 1 Recommendation. LOUCY cells showed enhanced sensitivity to palbociclib + nelfinavir as compared to non-ETP-ALL cell lines, KGI and Jurkat (Fig. 1 Although significant strides have be When a cell line possesses conflicted markers, the different combinations of alleles are computed and the Best and Worst reported as distinct rows with an additional label after their accession number. Product Reviews », Relevant content specific to your research interests. For instance, the Loucy cell line has a conflict for the D5S818 STR marker. COSMIC ID: 909260. For instance, the Loucy cell line has a conflict for the D5S818 STR marker. Culture harvest date THP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. Importantly, in Loucy cells, the only cell line responsive to IPI-145, proliferation was impaired already at 0.5 μM after treatment with this dual inhibitor (Fig. These permits may be required for shipping this product: ISO 17025 Search for genes and functional terms extracted and organized from over a hundred publicly available resources. As shown in Fig. Cell Type: PI3K pan-inhibition impairs proliferation in T-ALL cell lines. Cells from the Loucy cell line were preserved for 24 hours in Cell-Free DNA BCT reagent. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 10. LOUCY and HL60 cell lines were cultured in the absence or presence of DMSO (vehicle control) or KPT-330 (100 nM or 1000 nM) for 24 hr; cell lysates were prepared using RIPA buffer and analyzed by immunoblotting with anti-Nup214, anti-CRM1, or anti-GAPDH antibodies. Frozen In Stock. Description Established in 1987 from the peripheral blood of a patient with T-cell acute lymphoblastic leukemia (T-ALL) obtained two months prior to her death. A T-ALL cell line LOUCY (1/43 T cell lines tested) and an AML cell line MEGAL (1/53 myeloid cell lines tested) were the … Leuk. and new technologies for life scientists. Expression levels of lncRNA AWPPH and ROCK2 were detected 24 h after transfection. 4A, overexpression of lncRNA AWPPH and ROCK2 was achieved in the human T-ALL Loucy cell line (P<0.05; Fig. Cellosaurus RRID: CVCL_1380. It was found that phytohemagglutinin (PHA, 5 μmL) or PHA in combination with 12,13-phorbol dibutirate (PDBu, 10–8 M) increased the number of CD25-positive cells in the proliferating Jurkat culture. LOUCY, HL60, and K562 cell lines were cultured either with DMSO (vehicle control) or KPT330 (1000 nM) for 24 hr; cell lysates were prepared by boiling the cells in a sample buffer and analyzed by immunoblotting using anti-Nup214, anti-CRM1, or anti-GAPDH antibodies.